Coding

Part:BBa_K3619010:Design

Designed by: Isak Ingerholt   Group: iGEM20_Lund   (2020-09-13)


Gene NmDef02 encoding a antimicrobial putative defensin.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is codon optimized for expression in E. coli K12.


Source

NmDef02 was obtained from Nicotiana megalosiphon. DNA sequence was obtained through back translation of the peptide.

References

1. https://onlinelibrary.wiley.com/doi/full/10.1111/j.1467-7652.2010.00501.x

2. https://www.uniprot.org/uniprot/C5IJZ0